Meller D, Augustin A J, Koch F H
Department of Ophthalmology, University of Bonn, Germany.
Ophthalmic Res. 1996;28(2):71-9. doi: 10.1159/000267877.
In this article, the development and application of a modified technique of impression cytology for the electron-microscopic examination of corneal epithelium is presented. Corneal epithelium of controls and diabetic patients was obtained using millipore filters, which had been presoaked with EDTA to weaken intercellular adhesions. Light and electron microscopy (EM) of the control samples showed sheets of well-preserved corneal epithelial cells. Therefore, this modified technique is supposed to be useful for the analysis of alterations of the corneal epithelium. EM of corneal cells of diabetic patients showed irregularly formed epithelial cells. The corneal epithelium of diabetic patients revealed thickening of the basement membrane and a decreased number of hemidesmosomes. The alterations observed in specimens of diabetic patients represent the morphological equivalent of an impaired epithelial barrier function, an increased fragility of epithelial lining and the disturbed healing properties occurring in diabetic keratopathy.
本文介绍了一种改良的印片细胞学技术在角膜上皮细胞电镜检查中的开发与应用。使用经乙二胺四乙酸（EDTA）预浸泡以减弱细胞间黏附力的微孔滤膜获取对照组和糖尿病患者的角膜上皮细胞。对照组样本的光镜和电镜检查显示角膜上皮细胞片层保存良好。因此，这种改良技术有望用于分析角膜上皮的改变。糖尿病患者角膜细胞的电镜检查显示上皮细胞形态不规则。糖尿病患者的角膜上皮显示基底膜增厚和半桥粒数量减少。在糖尿病患者标本中观察到的这些改变相当于糖尿病角膜病变中上皮屏障功能受损、上皮衬里脆性增加以及愈合特性紊乱的形态学表现。
