Reifferscheid G, Heil J
AMMUG, University of Mainz, Germany.
Mutat Res. 1996 Aug 12;369(3-4):129-45. doi: 10.1016/s0165-1218(96)90021-x.
The present study gives a comprehensive update of all umu genotoxicity assay results published so far. The available data of 486 chemicals investigated with the umu test are compared with the Ames test (274 compounds) as well as rodent carcinogenicity data (179 compounds). On the whole, there is good agreement between the umu test and the Ames test results, with a concordance of about 90%. The umu test was able to detect 86% of the Ames mutagens, while the Ames test (using at least 5 strains) detected 97% of the umu positive compounds. The elimination of TA102 from the set of Ames tester strains reduced the percentage of detectable umu genotoxins from 97 to 86%. The agreement between carcinogenesis and umu response was 65%, which is comparable to earlier studies concerning rodent carcinogenesis and Salmonella mutagenesis. The present compilation of umu results provides a database that can be used for the comparison of the SOS-inducing activity of chemicals and their mutagenicity, respectively, carcinogenicity. The results presented here clearly demonstrate that a chemical which induces the expression of the umu operon can be regarded a rodent carcinogen with a high degree of certainty (93%).
本研究全面更新了迄今为止已发表的所有umu基因毒性试验结果。将用umu试验研究的486种化学物质的现有数据与艾姆斯试验（274种化合物）以及啮齿动物致癌性数据（179种化合物）进行了比较。总体而言，umu试验与艾姆斯试验结果之间具有良好的一致性，一致性约为90%。umu试验能够检测出86%的艾姆斯诱变剂，而艾姆斯试验（使用至少5种菌株）检测出了97%的umu阳性化合物。从艾姆斯试验菌株组中去除TA102后，可检测到的umu基因毒素的百分比从97%降至86%。致癌作用与umu反应之间的一致性为65%，这与早期关于啮齿动物致癌作用和沙门氏菌诱变作用的研究相当。目前umu结果的汇编提供了一个数据库，可分别用于比较化学物质的SOS诱导活性及其诱变性、致癌性。此处给出的结果清楚地表明，诱导umu操纵子表达的化学物质可以高度确定地（93%）被视为啮齿动物致癌物。
