Matsuoka A, Yamakage K, Kusakabe H, Wakuri S, Asakura M, Noguchi T, Sugiyama T, Shimada H, Nakayama S, Kasahara Y, Takahashi Y, Miura K F, Hatanaka M, Ishidate M, Morita T, Watanabe K, Hara M, Odawara K, Tanaka N, Hayashi M, Sofuni T
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan.
Mutat Res. 1996 Aug 12;369(3-4):243-52. doi: 10.1016/s0165-1218(96)90029-4.
In a collaborative study organized under the JEMS MMS, nine mouse lymphoma assay (MLA) "unique positive' NTP rodent carcinogens were re-evaluated by an in vitro chromosomal aberration assay using Chinese hamster lung fibroblast cells (CHL/IU). Six of nine chemicals induced chromosomal aberrations; bromodichloromethane, chlorendic acid and isophorone induced structural aberrations, and chlorodibromomethane, pentachloroethane and 1,1,1,2-tetrachloroethane induced numerical aberrations (polyploidy). These six chemicals, therefore, are not uniquely positive in the MLA. The difference between the NTP results and ours might be due to the use of different cell lines and protocols, and in some cases, to different interpretations of polyploidy. The remaining three chemicals, benzyl acetate, cinnamyl anthranilate and trichloroethylene, were negative in this study.
在由JEMS MMS组织的一项合作研究中，使用中国仓鼠肺成纤维细胞（CHL/IU）的体外染色体畸变试验对九种小鼠淋巴瘤试验（MLA）“独特阳性”的NTP啮齿动物致癌物进行了重新评估。九种化学物质中有六种诱导了染色体畸变；溴二氯甲烷、氯菌酸和异佛尔酮诱导了结构畸变，而氯二溴甲烷、五氯乙烷和1,1,1,2-四氯乙烷诱导了数量畸变（多倍体）。因此，这六种化学物质在MLA中并非独特阳性。NTP的结果与我们的结果之间的差异可能是由于使用了不同的细胞系和实验方案，在某些情况下，也可能是由于对多倍体的不同解释。其余三种化学物质，乙酸苄酯、邻氨基苯甲酸肉桂酯和三氯乙烯，在本研究中呈阴性。
