Härfast B, Johansson E, Johansson S G, van Hage-Hamsten M
Department of Laboratory Medicine, Karolinska Hospital, Stockholm, Sweden.
Allergy. 1996 Apr;51(4):257-61. doi: 10.1111/j.1398-9995.1996.tb04602.x.
ELISA (enzyme-linked immunosorbent assay) inhibition with a monoclonal antibody (mAb) (42B6) to Lepidoglyphus destructor was used to detect and quantify the storage-mite allergens in 30 dust samples collected from barns. Regarding the mite fauna, microscopic inspection of the barn dust and mite counts showed that L. destructor infested all 30 barns investigated (range 430-195 400 mites/g dust). In 29/30 barns, L. destructor constituted more than 70% of the Astigmata species. Acarus siro was found in 26 samples, the highest value being 16155 mites/g. No Dermatophagoides species were found. As to mites of the suborder of Prostigmata, species belonging to seven different families were detected. Besides the predominant L. destructor, allergens derived from other storage mites such as Glycyphagus domesticus, A. siro, and Tyrophagus putrescentiae have previously been assessed by this ELISA method. The correlation between number of mites and concentrations of mite antigen as measured by ELISA was assessed by linear regression (r2 = 0.83). Thus, inhibition of mAb 42B6 in ELISA would seem to offer a simple and reliable method to detect levels of dust-mite species belonging to the Acaridae and Glycyphagidae families.
采用针对粉螨的单克隆抗体（mAb）（42B6）进行的酶联免疫吸附测定（ELISA）抑制试验，来检测和定量从谷仓采集的30份灰尘样本中的仓储螨过敏原。关于螨类区系，对谷仓灰尘进行显微镜检查和螨计数显示，所有30个被调查的谷仓均受到粉螨侵染（范围为430 - 195400只螨/克灰尘）。在30个谷仓中的29个，粉螨占无气门目螨类的70%以上。在26个样本中发现了粗脚粉螨，最高值为16155只螨/克。未发现尘螨属物种。至于前气门亚目螨类，检测到属于七个不同科的物种。除了占主导地位的粉螨外，此前已通过这种ELISA方法评估了源自其他仓储螨的过敏原，如普通食甜螨、粗脚粉螨和腐食酪螨。通过线性回归评估螨数量与ELISA测定的螨抗原浓度之间的相关性（r2 = 0.83）。因此，ELISA中mAb 42B6的抑制作用似乎提供了一种简单可靠的方法来检测属于粉螨科和食甜螨科的尘螨物种水平。
