Guimond M J, Wang B, Fujita J, Terhorst C, Croy B A
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Canada.
Am J Reprod Immunol. 1996 Jun;35(6):501-9. doi: 10.1111/j.1600-0897.1996.tb00049.x.
Granulated metrial gland (GMG) cells are pregnancy-specific uterine lymphocytes found in rodents. The lineage relationships of GMG cells are incompletely defined, although genetic and immunophenotyping studies suggest membership in the natural killer (NK) cell lineage. Pregnancy-specific functions have been postulated for GMG cells, but no successful depletion of these cells has been achieved that would permit assessment of any critical roles that might influence gestational outcome.
Routine histological methods for light microscopy were used to assess implantation sites from wild-type mice and mice of the following genotypes: tgE26, mi/mi, and p53 knockout.
GMG cells are transient, histamine-negative cells found in the pregnant uteri of most mice. Pregnancies in the NK and T-cell-deficient tgE26 were characterized by infrequent GMG cells, very small placentae, and an elevated rate of fetal and perinatal mortality. In term placentae of mi/mi with dystocia. GMG cells were found in a new location along the plane of placental separation, and they appeared degranulated. A normal life-history was observed for GMG cells in p53 knockout mice.
Pregnancies in mutant and transgenic mice indicate that GMG cells are natural killer cells and have critical roles in placental development and pregnancy success. The disappearance of GMG cells at term is independent of p53 gene expression.
颗粒状子宫内膜腺（GMG）细胞是在啮齿动物中发现的妊娠特异性子宫淋巴细胞。尽管基因和免疫表型研究表明GMG细胞属于自然杀伤（NK）细胞谱系，但其谱系关系尚未完全明确。GMG细胞被认为具有妊娠特异性功能，但尚未成功清除这些细胞，因此无法评估其可能影响妊娠结局的任何关键作用。
使用常规组织学方法进行光学显微镜检查，以评估野生型小鼠以及以下基因型小鼠的着床部位：tgE26、mi/mi和p53基因敲除小鼠。
GMG细胞是大多数小鼠妊娠子宫中发现的短暂存在的、组胺阴性细胞。NK和T细胞缺陷的tgE26小鼠的妊娠特征为GMG细胞稀少、胎盘非常小以及胎儿和围产期死亡率升高。在难产的mi/mi小鼠足月胎盘中，GMG细胞出现在胎盘分离平面的一个新位置，且呈现脱颗粒状态。在p53基因敲除小鼠中观察到GMG细胞具有正常的生命周期。
突变和转基因小鼠的妊娠情况表明，GMG细胞是自然杀伤细胞，在胎盘发育和妊娠成功中具有关键作用。足月时GMG细胞的消失与p53基因表达无关。
