Tn和唾液酸化Tn抗原作为人类卵巢癌潜在的预后标志物。

Tn and sialyl-Tn antigens as potential prognostic markers in human ovarian carcinoma.

作者信息

Ogawa H, Ghazizadeh M, Araki T

机构信息

Department of Obstetrics and Gynecology, WHO Collaborating Center for Research and Training in Diagnostic Electron Microscopy, Nippon Medical School, Tokyo, Japan.

出版信息

Gynecol Obstet Invest. 1996;41(4):278-83. doi: 10.1159/000292284.

DOI:10.1159/000292284

PMID:8793500

Abstract

OBJECTIVE

Carcinoma-associated Tn and sialyl-Tn antigens have been shown to aid in the prediction of tumor aggressiveness. The purpose of this study was to evaluate the prognostic value of Tn and sialyl-Tn antigen expression in human ovarian carcinoma.

METHODS

Formalin-fixed, paraffin-embedded tissue sections from 32 primary ovarian carcinomas, 6 benign and 2 normal ovarian tissues were immunostained using monoclonal antibodies against Tn and sialyl-Tn antigens and a streptavidin-biotin-peroxidase method Immunostainings were assessed semiquantitatively and the measurements were then correlated with the established prognostic factors of ovarian carcinoma. i.e. disease stage and histological grade.

RESULTS

Of the 32 ovarian carcinomas, 22 (69%) expressed Tn and 28 (87.5%) sialyl-Tn antigens. Immunostaining measures for Tn antigen were significantly associated with the clinical stage (p < or = 0.02) and histological grade (p < or = 0.05) of the tumors. The results for sialyl-Tn antigen revealed more significant associations with the clinical stage (p < or = 0.002) and histological grade (p < or = 0.007). The clinical stage and histologic grade of the tumors were also highly correlated with each other. Similarly, combined Tn and sialyl-Tn antigen expression revealed significant correlations with the prognostic factors. Benign and normal ovarian tissues showed no reactive Tn and sialyl-Tn antigen.

CONCLUSIONS

The extent of Tn and sialyl-Tn antigen expression in primary ovarian carcinoma may contribute to the prognosis of the disease.

摘要

目的

癌相关Tn和唾液酸化Tn抗原已被证明有助于预测肿瘤侵袭性。本研究的目的是评估Tn和唾液酸化Tn抗原表达在人类卵巢癌中的预后价值。

方法

采用抗Tn和唾液酸化Tn抗原的单克隆抗体及链霉亲和素-生物素-过氧化物酶法，对32例原发性卵巢癌、6例良性卵巢组织和2例正常卵巢组织的福尔马林固定、石蜡包埋组织切片进行免疫染色。对免疫染色进行半定量评估，然后将测量结果与卵巢癌已确定的预后因素，即疾病分期和组织学分级进行关联分析。

结果

在32例卵巢癌中，22例（69%）表达Tn抗原，28例（87.5%）表达唾液酸化Tn抗原。Tn抗原的免疫染色测量结果与肿瘤的临床分期（p≤0.02）和组织学分级（p≤0.05）显著相关。唾液酸化Tn抗原的结果显示与临床分期（p≤0.002）和组织学分级（p≤0.007）的相关性更显著。肿瘤的临床分期和组织学分级之间也高度相关。同样，Tn和唾液酸化Tn抗原的联合表达与预后因素显著相关。良性和正常卵巢组织未显示反应性Tn和唾液酸化Tn抗原。