Handfield M, Simard P, Couillard M, Letarte R
Département de microbiologie, Faculté de médecine, Université Laval, Québec City, Canada.
Appl Environ Microbiol. 1996 Sep;62(9):3459-61. doi: 10.1128/aem.62.9.3459-3461.1996.
Aeromonas hydrophila isolated from food and drinking water was tested for pathogenicity by studying its hemolysis, hemagglutination, and cytotoxicity. Hemolysis, tested on erythrocytes from six different species, was more frequently seen with water isolates (64%) than with food isolates (48%). Hemagglutination was more frequently encountered with food isolates (92%) than with water isolates (73%). Cytotoxicity, evaluated on seven cell lines, was frequently observed with food isolates (92%) and with water isolates (73%). Heat treatment (56 degrees C for 10 min) of culture supernatant fluids inhibited the toxicity of some but not all toxin-producing isolates. Our results suggest that the human intestinal cell line HT-29 could be a useful complement for testing A. hydrophila exotoxins and for studying the enteropathogenicity of this species for humans.
从食品和饮用水中分离出的嗜水气单胞菌，通过研究其溶血、血凝和细胞毒性来检测其致病性。在六种不同物种的红细胞上进行溶血试验，水分离株（64%）比食品分离株（48%）更常出现溶血现象。食品分离株（92%）比水分离株（73%）更常出现血凝现象。在七种细胞系上评估细胞毒性，食品分离株（92%）和水分离株（73%）都经常观察到细胞毒性。培养上清液的热处理（56℃ 10分钟）可抑制部分而非全部产毒素分离株的毒性。我们的结果表明，人肠道细胞系HT-29可能是检测嗜水气单胞菌外毒素以及研究该菌对人类肠道致病性的有用补充。
