利用重复基因间DNA序列对致病性和病害抑制性链霉菌菌株进行分类。
Use of repetitive intergenic DNA sequences to classify pathogenic and disease-suppressive Streptomyces strains.
作者信息
Sadowsky M J, Kinkel L L, Bowers J H, Schottel J L
机构信息
Department of Soil, Water, and Climate, University of Minnesota, St. Paul 55108, USA.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3489-93. doi: 10.1128/aem.62.9.3489-3493.1996.
Abstract
PCR DNA fingerprinting using repetitive intergenic DNA sequences (rep-PCR) was investigated as a means of differentiating between closely related strains of Streptomyces which were, in some cases, indistinguishable by other classification methods. Our results demonstrated that the majority of strains had unique rep-PCR DNA fingerprints and established that the technique could be a very useful tool in rapidly determining strain identity.
摘要
利用重复基因间DNA序列进行PCR DNA指纹分析（rep-PCR），作为区分链霉菌密切相关菌株的一种手段进行了研究，在某些情况下，这些菌株用其他分类方法无法区分。我们的结果表明，大多数菌株具有独特的rep-PCR DNA指纹，并确定该技术在快速确定菌株身份方面可能是一种非常有用的工具。
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