Buck S L, Rosenthal R A
Department of Microbiology, Alcon Laboratories Inc., Fort Worth, Texas 76134-2099, USA.
Appl Environ Microbiol. 1996 Sep;62(9):3521-6. doi: 10.1128/aem.62.9.3521-3526.1996.
A standard methodology for quantitatively evaluating neutralizer toxicity against Acanthamoeba castellanii does not exist. The objective of this study was to provide a quantitative method for evaluating neutralizer toxicity against A. castellanii. Two methods were evaluated. A quantitative microtiter method for enumerating A. castellanii was evaluated by a 50% lethal dose endpoint method. The microtiter method was compared with the hemacytometer count method. A method for determining the toxicity of neutralizers for antimicrobial agents to A. castellanii was also evaluated. The toxicity to A. castellanii of Dey-Engley neutralizing broth was compared with Page's saline. The microtiter viable cell counts were lower than predicted by the hemacytometer counts. However, the microtiter method gives more reliable counts of viable cells. Dey-Engley neutralizing medium was not toxic to A. castellanii. The method presented gives consistent, reliable results and is simple compared with previous methods.
目前不存在用于定量评估中和剂对卡氏棘阿米巴毒性的标准方法。本研究的目的是提供一种定量方法来评估中和剂对卡氏棘阿米巴的毒性。对两种方法进行了评估。通过50%致死剂量终点法评估了一种用于计数卡氏棘阿米巴的定量微量滴定法。将微量滴定法与血细胞计数器计数法进行了比较。还评估了一种确定抗菌剂中和剂对卡氏棘阿米巴毒性的方法。比较了戴伊 - 恩格利中和肉汤与佩奇氏生理盐水对卡氏棘阿米巴的毒性。微量滴定法的活细胞计数低于血细胞计数器计数的预测值。然而，微量滴定法能给出更可靠的活细胞计数。戴伊 - 恩格利中和培养基对卡氏棘阿米巴无毒。所提出的方法给出了一致、可靠的结果，并且与以前的方法相比更简单。
