Hayashi S, Guex-Crosier Y, Delvaux A, Velu T, Roberge F G
National Eye Institute, National Institutes of Health, Bethesda, MD 20892-1858, USA.
Graefes Arch Clin Exp Ophthalmol. 1996 Oct;234(10):633-6. doi: 10.1007/BF00185297.
Endotoxin-induced uveitis (EIU) is a model for acute anterior uveitis associated with a variety of pro-inflammatory cytokines and nitric oxide production. Interleukin 10 (IL-10) down-regulates these inflammatory mediators. We report a study of the effect of systemic administration of IL-10 on the inflammatory parameters of EIU.
Uveitis was induced in C3H/HeN mice by subcutaneous injection of 200 micrograms lipopolysaccharide (LPS) per mouse. Intraocular inflammation was assessed by leukocyte count and measurement of the protein concentration in the aqueous humor (AH). Mouse recombinant IL-10 at 1000 U or its vehicle alone were administered by three intravenous injections given 4.0 h and 0.5 h before and 8.0 h after LPS injection.
The inflammatory cell infiltration in the eyes was significantly reduced in four of five experiments from 40% to 64% in the groups treated with IL-10 compared to the control groups (P < 0.05). In contrast, the level of protein exudation in the anterior chamber (AC) was not significantly affected by IL-10 treatment.
IL-10 reduces the cellular infiltration in the ocular inflammation produced by endotoxin. This result suggests potential usefulness for IL-10 in the treatment of severe anterior uveitis with a strong cellular component.
内毒素诱导的葡萄膜炎（EIU）是一种与多种促炎细胞因子和一氧化氮产生相关的急性前葡萄膜炎模型。白细胞介素10（IL-10）可下调这些炎症介质。我们报告了一项关于全身给予IL-10对EIU炎症参数影响的研究。
通过每只小鼠皮下注射200微克脂多糖（LPS）在C3H/HeN小鼠中诱导葡萄膜炎。通过白细胞计数和测量房水（AH）中的蛋白质浓度来评估眼内炎症。在LPS注射前4.0小时和0.5小时以及注射后8.0小时通过三次静脉注射给予1000 U小鼠重组IL-10或仅给予其载体。
与对照组相比，在五个实验中的四个实验中，用IL-10治疗的组眼中的炎症细胞浸润从40%显著降低至64%（P<0.05）。相比之下，IL-10治疗对前房（AC）中的蛋白质渗出水平没有显著影响。
IL-10可减少内毒素引起的眼部炎症中的细胞浸润。该结果表明IL-10在治疗具有强烈细胞成分的严重前葡萄膜炎中具有潜在的应用价值。
