Navarro J A, Caro M R, Seva J, Rosillo M C, Gomez M A, Gallego M C
U.D. Histology and Anatomic Pathology, Veterinary Faculty, University of Murcia, Spain.
Vet Immunol Immunopathol. 1996 May;51(1-2):147-56. doi: 10.1016/0165-2427(95)05497-9.
Monoclonal antibodies (mAbs) to surface markers of bovine lymphocytes MHC I, MHC II, B-cells, T-cells (CD2, CD4, CD8 and gamma/delta) and interleukin-2 (IL-2) receptor were tested in the goat by flow cytometry and using immunohistochemical methods. Samples from peripheral blood and secondary lymphoid organs (mesenteric lymph nodes, spleen and ileal Peyer's patch) were studied. The percentage of positive cells obtained by flow cytometry and its compartmentalisation in different tissue sections showed that the mAbs against MHC I, MHC II, CD2, CD4, CD8, gamma/delta and IL-2 receptor recognised lymphocyte subpopulations similar to those present in the bovine. However, the mAbs tested on B-cells reacted only partially in the recognition of this subpopulation.
利用流式细胞术和免疫组织化学方法，在山羊中检测了针对牛淋巴细胞表面标志物MHC I、MHC II、B细胞、T细胞（CD2、CD4、CD8和γ/δ）以及白细胞介素-2（IL-2）受体的单克隆抗体（mAb）。研究了外周血和二级淋巴器官（肠系膜淋巴结、脾脏和回肠派伊尔结）的样本。通过流式细胞术获得的阳性细胞百分比及其在不同组织切片中的分布情况表明，针对MHC I、MHC II、CD2、CD4、CD8、γ/δ和IL-2受体的单克隆抗体识别的淋巴细胞亚群与牛体内的相似。然而，在B细胞上测试的单克隆抗体在识别该亚群时仅产生部分反应。
