Shyjan A M, Heldin P, Butcher E C, Yoshino T, Briskin M J
LeukoSite Inc., Cambridge, Massachusetts 02142, USA.
J Biol Chem. 1996 Sep 20;271(38):23395-9. doi: 10.1074/jbc.271.38.23395.
Hyaluronan is a constituent of the extracellular matrix of connective tissue and is actively synthesized during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of hyaluronan are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, hyaluronan has been implicated as an important substrate for migration of adhesion of leukocytes during inflammation. A human hyaluronan synthase (HuHAS1) cDNA was isolated by a functional expression cloning approach. Transfection of CHO cells conferred hyaluronidase-sensitive adhesiveness of a mucosal T cell line via the lymphocyte hyaluronan receptor, CD44, as well as increased hyaluronan levels in the cultures of transfected cells. The HuHAS1 amino acid sequence shows considerable homology to the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase from Xenopus laevis (DG42), and is the human homolog of a recently described murine hyaluronan synthase.
透明质酸是结缔组织细胞外基质的一种成分，在伤口愈合和组织修复过程中会被积极合成，为血管和成纤维细胞的向内生长提供框架。透明质酸血清浓度的变化与类风湿关节炎等炎症性和退行性关节病有关。此外，透明质酸被认为是炎症期间白细胞迁移和黏附的重要底物。通过功能表达克隆方法分离出了人透明质酸合酶（HuHAS1）cDNA。CHO细胞的转染通过淋巴细胞透明质酸受体CD44赋予了黏膜T细胞系对透明质酸酶敏感的黏附性，同时也提高了转染细胞培养物中的透明质酸水平。HuHAS1氨基酸序列与化脓性链球菌的hasA基因产物、非洲爪蟾的一种糖胺聚糖合成酶（DG42）具有相当的同源性，并且是最近描述的鼠透明质酸合酶的人类同源物。
