Borden P, Solymar D, Sucharczuk A, Lindman B, Cannon P, Heller R A
Roche Bioscience, Palo Alto, California 94304, USA.
J Biol Chem. 1996 Sep 20;271(38):23577-81. doi: 10.1074/jbc.271.38.23577.
Human collagenase-3 expression, previously seen only in a breast tumor tissue, is here shown to be expressed in primary human chondrocytes derived from the joint tissue and in transformed human chondrocytes. Its mRNA is inducible by the inflammatory cytokines interleukin-1beta plus tumor necrosis factor-alpha, but not by phorbol 12-myristate 13-acetate and only slightly by the growth factors platelet-derived growth factor and epidermal growth factor. Human synovial fibroblasts, another prominent cell type in the joint tissue, do not produce collagenase-3 message. Expression of the murine collagenase, which is possibly the counterpart of human collagenase-3, is induced by interleukin-1beta plus tumor necrosis factor-alpha, and its full induction requires the presence of the transcription factor, c-FOS. This family of transcription factors also plays a role in induction of human collagenase-3, since it binds to the AP-1 site of this matrix metalloproteinase.
人胶原酶-3的表达，以前仅在乳腺肿瘤组织中可见，现发现其在源自关节组织的原代人软骨细胞和转化的人软骨细胞中表达。其信使核糖核酸（mRNA）可被炎性细胞因子白细胞介素-1β加肿瘤坏死因子-α诱导，但不能被佛波酯12-肉豆蔻酸酯13-乙酸酯诱导，仅被生长因子血小板衍生生长因子和表皮生长因子轻微诱导。人滑膜成纤维细胞是关节组织中另一种主要细胞类型，不产生胶原酶-3信使。小鼠胶原酶的表达可能与人胶原酶-3相对应，可被白细胞介素-1β加肿瘤坏死因子-α诱导，其完全诱导需要转录因子c-FOS的存在。该转录因子家族在人胶原酶-3的诱导中也起作用，因为它与这种基质金属蛋白酶的AP-1位点结合。
