与哺乳动物胞嘧啶DNA甲基转移酶基因同源的黑腹果蝇基因组DNA序列。

Drosophila melanogaster genomic DNA sequence homologous to mammalian cytosine DNA-methyltransferase gene.

作者信息

Vanyushin B F, Poirier L A

机构信息

Division of Nutritional Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, USA.

出版信息

Biochem Mol Biol Int. 1996 May;39(2):353-8. doi: 10.1080/15216549600201381.

DOI:10.1080/15216549600201381

PMID:8799463

Abstract

Using Southern blotting procedure we have shown that Drosophila genomic DNA hybridizes with 4423-bp C-terminal fragment of murine cytosine DNA-methyltransferase gene. Thus, the Drosophila genome has a sequence homologous to the mammalian cytosine DNA-methyltransferase gene. We assume that DNA methylation most likely responsible for strong CpG suppression in the Drosophila genome mainly was catalyzed by a cytosine DNA-methyltransferase that has since been lost.

摘要

通过Southern杂交技术，我们发现果蝇基因组DNA与小鼠胞嘧啶DNA甲基转移酶基因的4423bp C末端片段发生杂交。因此，果蝇基因组具有与哺乳动物胞嘧啶DNA甲基转移酶基因同源的序列。我们推测，果蝇基因组中导致强烈CpG抑制的DNA甲基化很可能主要由一种现已丢失的胞嘧啶DNA甲基转移酶催化。