Cavicchini S, Brezzi A, Gasparini G, Caputo R
Institute of Dermatological Sciences, University of Milan, IRCCS Ospedale Maggiore, Italy.
Acta Derm Venereol. 1996 May;76(3):186-9. doi: 10.2340/0001555576186189.
Electron microscopic details in psoriatic skin during calcipotriol application are reported. Ten psoriatic patients (PASI score 3-14) were punch-biopsied on typical lesions before treatment and 2, 4 and 8 weeks after it. Two patients dropped out after 1 month because of lack of clinical response. Skin blocks were processed routinely for transmission electron microscopy and freeze-fracture techniques. The first modifications were the disappearance of fine intercellular granular material and the restoration of the granular layer. After 4 weeks desmosomes appeared in greater amounts than at baseline. Horny layer lipid vacuoles and remnants of nuclei as well as multilayering of the basal lamina were only focally observed. Dilatation of dermal capillaries was still seen even after 8 weeks. Freeze-fracture confirmed the data about the desmosomes and revealed a marked decrease of the gap junctions. Calcipotriol basically seems to act similarly to other antipsoriatic agents, though faster. The persistence of microvasculature dilatation could imply the need for long-term therapy.
报告了应用卡泊三醇期间银屑病皮肤的电子显微镜细节。对10例银屑病患者（银屑病面积和严重程度指数评分3 - 14）在治疗前及治疗后2周、4周和8周对典型皮损进行钻孔活检。2例患者在1个月后因缺乏临床反应退出。皮肤组织块常规进行透射电子显微镜和冷冻断裂技术处理。最初的改变是细胞间细微颗粒物质消失以及颗粒层恢复。4周后桥粒数量比基线时增多。角质层脂质空泡、细胞核残余以及基底膜多层化仅在局部观察到。即使在8周后仍可见真皮毛细血管扩张。冷冻断裂证实了关于桥粒的数据，并显示缝隙连接明显减少。卡泊三醇的作用基本与其他抗银屑病药物相似，不过起效更快。微血管扩张持续存在可能意味着需要长期治疗。
