Keller H U, Bebie H
Institute of Pathology, University of Bern, Switzerland.
Cell Motil Cytoskeleton. 1996;33(4):241-51. doi: 10.1002/(SICI)1097-0169(1996)33:4<241::AID-CM1>3.0.CO;2-C.
Locomoting blebbing cells have been used as a model to obtain novel insight into the mechanisms of cell locomotion. We tested the hypothesis that locomotion can be due to progressive one-sided protrusion of cellular volume into pseudopods. The hypothesis is supported by the finding that the rate and direction of locomotion of individual Walker carcinosarcoma cells can be predicted by sequential measurement of protrusive activity. Protrusive activity at the front is closely associated with forward movement of the rear part of the cell. During bleb formation the cell membrane of Walker carcinosarcoma cells is pushed forward faster (1.2-4.1 microns/sec) than known rates of actin elongation.
具有运动性的气泡状细胞已被用作一种模型，以获得对细胞运动机制的新见解。我们检验了这样一种假说，即运动可能是由于细胞体积向伪足的渐进性单侧突出所致。这一假说得到了以下发现的支持：通过对突出活动的连续测量，可以预测单个沃克癌肉瘤细胞的运动速率和方向。细胞前端的突出活动与细胞后部的向前运动密切相关。在气泡形成过程中，沃克癌肉瘤细胞的细胞膜向前推进的速度（1.2 - 4.1微米/秒）比已知的肌动蛋白伸长速度要快。
