Speed B R, Kaldor J, Cairns B, Pegorer M
Medical Division, Fairfield Hospital, Victoria, Australia.
J Med Vet Mycol. 1996 Jun-Jul;34(3):187-93.
Using a class-specific enzyme immunoassay IgG and IgA anticryptococcal antibody was measured in 400 serum specimens at 1 week to 11.7 years after diagnosis from 43 immunocompetent subjects with confirmed active cryptococcosis. The prevalence of IgG was 86% at diagnosis, rose to 100% by 2 weeks and remained high thereafter. IgA prevalence was 71% at diagnosis, rose to 75% at 2 weeks and then fell over 2 years. IgG and IgA prevalence in paediatric controls was 5% and 0%, respectively. Mean antibody levels showed the same pattern and neither levels nor prevalence were influenced by age, sex or site of infection. Patients with the variety gattii infection had a greater antibody response than those with the variety neoformans which was significant for IgA. Specific anticryptococcal antibody was regularly present in conjunction with cryptococcal antigen at diagnosis. IgG persists but IgA falls over 1-2 years. The assay described may be a useful tool to study the antibody response and seroepidemiology of infection with C. neoformans.
采用针对特定类别的酶免疫测定法，对43例确诊为活动性隐球菌病的免疫功能正常受试者在诊断后1周11.7年的400份血清样本检测了IgG和IgA抗隐球菌抗体。诊断时IgG的阳性率为86%，2周时升至100%，此后保持在较高水平。IgA阳性率在诊断时为71%，2周时升至75%，然后在2年内下降。儿科对照中IgG和IgA的阳性率分别为5%和0%。平均抗体水平呈现相同模式，抗体水平和阳性率均不受年龄、性别或感染部位的影响。加蒂变种感染患者的抗体反应比新生变种感染患者更强，这在IgA方面具有显著性差异。在诊断时，特异性抗隐球菌抗体常与隐球菌抗原同时出现。IgG持续存在，但IgA在12年内下降。所描述的检测方法可能是研究新生隐球菌感染的抗体反应和血清流行病学的有用工具。
