嗜酒明串珠菌（酒类酒球菌）苹果酸-乳酸发酵途径的体外重组
In vitro reassembly of the malolactic fermentation pathway of Leuconostoc oenos (Oenococcus oeni).
作者信息
Salema M, Capucho I, Poolman B, San Romão M V, Dias M C
机构信息
Instituto de Technologia Química e Biológica, U.N.L., Oeiras, Portugal.
出版信息
J Bacteriol. 1996 Sep;178(18):5537-9. doi: 10.1128/jb.178.18.5537-5539.1996.
The mechanism of metabolic energy generation by malolactic fermentation was studied with artificial membrane vesicles of Leuconostoc oenos (Oenococcus oeni). (Note that although L. oenos was recently reclassified as O. oeni [L. M. T. Dicks, F. Dellaglio, and M. D. Collins, Int. J. Syst. Bacteriol. 45:395-397, 1995], the old designation was kept in the present work.) Purified malolactic enzyme was entrapped in artificial membrane vesicles prepared from L. oenos cells able to transport L-malate. We show that the in vitro reconstituted system, including an electrogenic L-malate carrier and the decarboxylating malolactic enzyme, generated a proton motive force that was able to drive intravesicular accumulation of leucine.
利用嗜杀片球菌（酒类酒球菌）的人工膜囊泡研究了苹果酸-乳酸发酵产生代谢能量的机制。（注意，尽管嗜杀片球菌最近被重新分类为酒类酒球菌[L.M.T.迪克斯、F.德拉廖和M.D.柯林斯，《国际系统细菌学杂志》45:395 - 397，1995年]，但在本研究中仍保留旧的命名。）将纯化的苹果酸-乳酸酶包封在由能够转运L-苹果酸的嗜杀片球菌细胞制备的人工膜囊泡中。我们发现，体外重建系统，包括一个生电L-苹果酸载体和脱羧苹果酸-乳酸酶，产生了一个质子动力势，该质子动力势能够驱动亮氨酸在囊泡内积累。
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