Ismail H I, Hashimoto Y, Kon Y, Okada K, Davis W C, Iwanaga T
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Vet Immunol Immunopathol. 1996 Jul;52(3):201-12. doi: 10.1016/0165-2427(96)05552-3.
Mammary glands of pregnant, lactating and resting goats were studied by immunohistochemistry for lymphocyte subpopulations using a panel of monoclonal antibodies. All T lymphocyte subpopulations that may have a role in the immune response, CD2+, CD4+, CD8+ and gamma delta T cells and subsets, were present in the mammary gland and were noted to increase in number progressively during pregnancy, decrease significantly during lactation, and then moderately increase during the resting period. CD4+ cells, the predominant cell type in the mammary gland, were located mainly in the connective tissue, whereas CD2+, CD8+ and TcR1-N24+ cells were predominant in the intraepithelial areas. TcR1-N6+ cells were detected almost exclusively during pregnancy, being localized mainly in the connective tissue. Their proportion decreased markedly following parturition. Very few WC1-N3+ and -N4+ cells were detected in the mammary gland. It is suggested that the majority of gamma delta T lymphocytes in the mammary gland of the goat are CD2+ CD8+ WCl-, a distinctive subset from that of the WCl+ subset in peripheral blood.
利用一组单克隆抗体，通过免疫组织化学方法对怀孕、泌乳和休情期山羊的乳腺进行淋巴细胞亚群研究。所有可能在免疫反应中起作用的T淋巴细胞亚群，即CD2⁺、CD4⁺、CD8⁺和γδT细胞及其亚群，均存在于乳腺中，且在怀孕期间数量逐渐增加，在泌乳期显著减少，然后在休情期适度增加。CD4⁺细胞是乳腺中的主要细胞类型，主要位于结缔组织中，而CD2⁺、CD8⁺和TcR1 - N24⁺细胞在上皮内区域占主导。TcR1 - N6⁺细胞几乎仅在怀孕期间检测到，主要定位于结缔组织中。分娩后其比例显著下降。在乳腺中检测到极少的WC1 - N3⁺和 - N4⁺细胞。研究表明，山羊乳腺中的大多数γδT淋巴细胞是CD2⁺CD8⁺WCl⁻，这是一个与外周血中WCl⁺亚群不同的独特亚群。
