Chabaudie N, Le Jan C, Olivier M, Salmon H
Res Vet Sci. 1993 Nov;55(3):351-5. doi: 10.1016/0034-5288(93)90106-p.
The presence and localisation of lymphocyte subsets together with class II bearing cells in the mammary gland of sows, were studied at different periods of the reproductive cycle by immunohistochemistry and compared with blood. All cell types involved in the immune response were present in the mammary gland at the different stages of gestation and lactation and nearer the alveolar epithelium as gestation proceeded: T lymphocytes, including CD4+ and CD8+, B lymphocytes and class II bearing cells (epithelial cells and macrophages). The results indicated an early accumulation of T lymphocytes, specifically T helper cells, during pregnancy; the specific increase of IgA lymphocytes occurring after this phase could suggest a role for these T cells in the induction of IgA response. The local accumulation of immune cells sustains the view that the mammary gland is able to mount a true local immune response and the increase in CD8+ cells near the epithelium suggests a role in local immune defence.
通过免疫组织化学方法，研究了母猪乳腺中淋巴细胞亚群以及Ⅱ类分子阳性细胞的存在情况和定位，并在生殖周期的不同阶段与血液进行了比较。在妊娠和哺乳的不同阶段，乳腺中均存在参与免疫反应的所有细胞类型，并且随着妊娠的进展，这些细胞更靠近肺泡上皮：包括CD4⁺和CD8⁺的T淋巴细胞、B淋巴细胞以及Ⅱ类分子阳性细胞（上皮细胞和巨噬细胞）。结果表明，在妊娠期间T淋巴细胞，特别是辅助性T细胞早期积累；在此阶段之后出现的IgA淋巴细胞特异性增加，可能表明这些T细胞在诱导IgA反应中发挥作用。免疫细胞的局部积累支持了乳腺能够产生真正局部免疫反应的观点，上皮附近CD8⁺细胞的增加表明其在局部免疫防御中的作用。
