Taliansky M E, Robinson D J, Murant A F
Scottish Crop Research Institute, Invergowrie, Dundee, UK.
J Gen Virol. 1996 Sep;77 ( Pt 9):2335-45. doi: 10.1099/0022-1317-77-9-2335.
Complementary DNA clones representing the entire genome of groundnut rosette umbravirus (GRV) were obtained and sequenced. GRV RNA comprises 4019 nucleotides and contains four large open reading frames (ORFs). The second ORF from the 5' end includes sequences that encode motifs characteristic of viral RNA-dependent RNA polymerases and is probably expressed by a -1 frameshift mechanism as a fusion protein with the product of the 5'-most ORF. The other two ORFs are almost completely overlapping in different reading frames, and are probably expressed from subgenomic RNA. One of the putative products has significant sequence similarity with viral movement proteins. None of the putative proteins encoded by GRV RNA seems to be a structural protein. In genome organization and in the amino acid sequences of its potential products, the RNA of GRV is similar to that of carrot mottle mimic umbravirus, and to the umbravirus-like RNA-2 of pea enation mosaic virus.
获得了代表花生丛簇伞形病毒（GRV）全基因组的互补DNA克隆并进行了测序。GRV RNA由4019个核苷酸组成，包含四个大的开放阅读框（ORF）。从5'端起的第二个ORF包含编码病毒RNA依赖性RNA聚合酶特征基序的序列，可能通过-1移码机制作为与最5'端ORF产物的融合蛋白表达。另外两个ORF在不同阅读框中几乎完全重叠，可能从亚基因组RNA表达。其中一个推定产物与病毒运动蛋白具有显著的序列相似性。GRV RNA编码的推定蛋白似乎都不是结构蛋白。在基因组组织及其潜在产物的氨基酸序列方面，GRV的RNA与胡萝卜斑驳拟态伞形病毒以及豌豆耳突花叶病毒的类伞形病毒RNA-2相似。
