Miller P H, Facklam R R, Miller J M
Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1996 Apr;34(4):1027-8. doi: 10.1128/jcm.34.4.1027-1028.1996.
The growth of Alloiococcus otitis under different atmospheres and nutritional conditions was studied. The growth rates of 25 strains of gram-positive cocci representing five genera on heart infusion agar plates containing 5% rabbit blood and on brucella agar plates with and without sheep blood under aerobic, increased CO2, and anaerobic atmospheres were compared. Eight strains of alloiococci plated on heart infusion agar with rabbit blood and on brucella sheep blood agar grew under aerobic and candle jar atmospheres. Two of these strains showed poor anaerobic growth after 7 days. Strains of Aerococcus viridans, Aerococcus urinae, Helcococci kunzi, Dolosigranulum pigrum, Gemella haemolysans, and Gemella morbillorum grew well under all three atmospheres and on the three types of media and in thioglycolate broth. These results confirm all the aerobic atmospheric requirements for Alloiococcus strains and show that aerobic growth characteristics help distinguish the alloiococci from the other gram-positive cocci that are facultatively anaerobic.
研究了不同大气环境和营养条件下耳炎差异球菌的生长情况。比较了代表五个属的25株革兰氏阳性球菌在含5%兔血的心浸液琼脂平板上以及在有氧、增加二氧化碳和厌氧环境下有无羊血的布鲁氏菌琼脂平板上的生长速率。接种在含兔血的心浸液琼脂和布鲁氏菌羊血琼脂上的8株差异球菌在有氧和烛缸环境下生长。其中两株在7天后厌氧生长较差。绿色气球菌、尿液气球菌、昆氏解糖嗜胨菌、颗粒链菌属、溶血孪生球菌和麻疹孪生球菌在所有三种环境下、在三种类型的培养基上以及硫乙醇酸盐肉汤中均生长良好。这些结果证实了差异球菌菌株对需氧大气的所有要求，并表明需氧生长特性有助于将差异球菌与其他兼性厌氧的革兰氏阳性球菌区分开来。
