Pao C C, Yang F P, Lai Y M
Chang Gung College of Medicine and Technology, Taipei, Taiwan, Republic of China.
Fertil Steril. 1996 Oct;66(4):630-3. doi: 10.1016/s0015-0282(16)58580-x.
To determine if sperm cells differentially take up or retain different regions of human papilloma virus (HPV) type 18 genome.
A descriptive clinical study.
A major medical center affiliated with a medical college.
Twenty-three randomly selected patients who attended Fertility Clinics at the Chang Gung Memorial Hospital. Semen specimens were obtained from volunteered patients who attended fertility clinics at the Chang Gung Memorial Hospital.
The presence of various regions of HPV type 18 genome in sperm cells was determined by polymerase chain reaction.
Among 23 sperm specimens that were positive for HPV type 18 DNA by polymerase chain reaction, the upstream regulatory region, E6, E7, E1, and L1 regions or open region frames were detected in 4 (17%), 7 (30%), 19 (83%), 5 (22%), and 1 (4%) specimens, respectively. The differential display of the presence of various regions of the HPV type 18 genome was not the result of different amplification and detection efficiencies of these DNA fragments.
These results suggest that the oncogenic portion of HPV DNA is present in spermatozoa. Furthermore, the E6 and E7 regions of the viral genome preferentially were taken up and/or retained by the human sperm cells.
确定精子细胞是否差异摄取或保留人乳头瘤病毒18型（HPV-18）基因组的不同区域。
一项描述性临床研究。
一所医学院附属的大型医疗中心。
随机选择的23名就诊于长庚纪念医院生育门诊的患者。精液标本取自自愿就诊于长庚纪念医院生育门诊的患者。
采用聚合酶链反应测定精子细胞中人乳头瘤病毒18型基因组各区域的存在情况。
在23份经聚合酶链反应检测HPV-18 DNA呈阳性的精子标本中，分别在4份（17%）、7份（30%）、19份（83%）、5份（22%）和1份（4%）标本中检测到上游调控区、E6、E7、E1和L1区或开放阅读框。HPV-18基因组各区域存在情况的差异显示并非这些DNA片段不同扩增和检测效率的结果。
这些结果表明，HPV DNA的致癌部分存在于精子中。此外，病毒基因组的E6和E7区域优先被人类精子细胞摄取和/或保留。
