Angelopoulos T, Moshel Y A, Lu L, Macanas E, Grifo J A, Krey L C
Department of Obstetrics and Gynecology, New York University Medical Center, New York 10016, USA.
Fertil Steril. 1998 Apr;69(4):740-7. doi: 10.1016/s0015-0282(98)00016-8.
To look for correlations between acridine orange (AO) staining and semen parameters before and after sperm separation procedures and to assess whether the AO test predicts fertilization or pregnancy outcomes after standard IVF and intracytoplasmic sperm injection.
Prospective study that simultaneously assesses sperm morphology and nuclear protein maturity on a cell-by-cell basis before and after preparative procedures.
University teaching hospital.
PATIENT(S): Men (n = 140) undergoing diagnostic semen analysis.
MAIN OUTCOME MEASURE(S): Acridine orange fluorescence of sperm nuclei, semen parameters, IVF outcome.
RESULT(S): In unprocessed samples, 90% of sperm with normal heads displayed green fluorescence (mature nuclear protein); significantly lower percentages of green fluorescence were observed in sperm with abnormal heads. The percentage of mature normal sperm in the specimen correlated with motility. Sperm maturity after swim-up or Percoll gradient was significantly improved for sperm with normal or abnormal heads. The percentage of mature normal sperm correlated with motility after either Percoll or swim-up. Neither the percentages of mature nuclei nor mature normal nuclei correlated with fertilization or pregnancy outcome.
CONCLUSION(S): Nuclear protein maturation correlates with sperm motility and morphology. Because morphologically normal and motile sperm are more mature, separation procedures should generate a population of sperm with the highest fertilization capacity. Acridine orange staining, however, did not predict fertilization efficiency or pregnancy outcome in IVF cycles.
寻找吖啶橙（AO）染色与精子分离程序前后精液参数之间的相关性，并评估AO检测能否预测标准体外受精（IVF）和卵胞浆内单精子注射（ICSI）后的受精或妊娠结局。
前瞻性研究，在制备程序前后逐细胞评估精子形态和核蛋白成熟度。
大学教学医院。
接受诊断性精液分析的男性（n = 140）。
精子细胞核的吖啶橙荧光、精液参数、IVF结局。
在未经处理的样本中，90%头部正常的精子显示绿色荧光（成熟核蛋白）；头部异常的精子中观察到的绿色荧光百分比显著较低。样本中成熟正常精子的百分比与活力相关。对于头部正常或异常的精子，上浮或Percoll梯度处理后的精子成熟度显著提高。Percoll或上浮处理后，成熟正常精子的百分比与活力相关。成熟细胞核或成熟正常细胞核的百分比均与受精或妊娠结局无关。
核蛋白成熟与精子活力和形态相关。由于形态正常且有活力的精子更成熟，分离程序应产生具有最高受精能力的精子群体。然而，吖啶橙染色不能预测IVF周期中的受精效率或妊娠结局。
