Surani M A
J Reprod Fertil. 1977 Jul;50(2):289-96. doi: 10.1530/jrf.0.0500289.
Radiolabelled uterine luminal proteins were obtained 6 h after instillation of 5--10 muCi [35S]methionine into the lumen of each uterine horn of rats. The proteins were analysed electrophoretically on 6% polyacrylamide gels and incorporation of the precursor into the separated proteins was estimated in 0-18 cm gel slices by scintillation spectrometry. Major incorporation was obtained on Day 5 of pregnancy in a group of proteins of approximate mol. wt 3 X 10(4), 4-5 X 10(4) and 5-5 X 10(4) and in proteins of mol. wt 8--10 X 10(4). During pro-oestrus, substantial incorporation of the precursor occurred in a group of protein of mol, wt 13-5 X 10(4), 12-5 X 10(4) and 7-5--8-0 X 10(4) and slight incorporation in proteins of mol. wt 4-5 X 10(4). These changes were mimicked in ovariectomized females by injections of appropriate steroids: progesterone and oestradiol were necessary to induce the proteins detected during pregnancy, oestradiol alone was able to reproduce the changes observed at pro-oestrus. The proteins in the lumen appear to be of uterine origin because radiolabelled serum proteins were not detected in the lumen after an i.p. injection of the isotope. Also, labelled proteins were detected in the horn receiving the isotope but not in the contralateral horn injected with saline. Cycloheximide, given i.p. simultaneously with the isotope but not in the contralateral horn injected with saline. Cycloheximide, given i.p. simultaneously with the isotope, suppressed synthesis of uterine proteins.
向大鼠每个子宫角腔内注入5 - 10微居里的[35S]甲硫氨酸6小时后，获取放射性标记的子宫腔蛋白。这些蛋白在6%聚丙烯酰胺凝胶上进行电泳分析，通过闪烁光谱法在0 - 18厘米的凝胶切片中估算前体掺入分离蛋白的情况。在妊娠第5天，分子量约为3×10⁴、4 - 5×10⁴和5 - 5×10⁴的一组蛋白以及分子量为8 - 10×10⁴的蛋白中有大量掺入。在发情前期，前体大量掺入分子量为13 - 5×10⁴、12 - 5×10⁴和7 - 5 - 8 - 0×10⁴的一组蛋白中，而分子量为4 - 5×10⁴的蛋白中有少量掺入。在去卵巢的雌性大鼠中，注射适当的类固醇可模拟这些变化：孕酮和雌二醇是诱导妊娠期间检测到的蛋白所必需的，单独使用雌二醇就能重现发情前期观察到的变化。腔内的蛋白似乎来源于子宫，因为腹腔注射同位素后，腔内未检测到放射性标记的血清蛋白。此外，在接受同位素的子宫角中检测到标记蛋白，而在注射生理盐水的对侧子宫角中未检测到。腹腔内同时注射环己酰亚胺和同位素，但在注射生理盐水的对侧子宫角中未注射。腹腔内同时注射同位素时，环己酰亚胺抑制子宫蛋白的合成。
