Surani M A, Webb F T
J Endocrinol. 1977 Sep;74(3):431-9. doi: 10.1677/joe.0.0740431.
An injection of 12-5 nmol dibutyryl cyclic AMP (dbcAMP) into each uterine horn of ovariectomized rats maintained on progesterone alone, enhanced incorporation of [3H]leucine into uterine luminal proteins. A value of 11-73 X 10(3) c.p.m. [3H]leucine incorporated/rat was found after injection of dbcAMP compared with 3-69 X 10(3) c.p.m./rat after injection of saline. An injection of 1-0 microgram oestradiol, in contrast, gave 58-05 X 10(3) c.p.m./rat. Electrophoretic analysis showed that the radioactively labelled proteins found after an injection of dbcAMP or oestradiol were qualitatively similar to those detected on day 5 of pregnancy, the day of blastocyst implantation. The nucleotide thus apparently elicits a uterine response similar to that observed after an injection of oestradiol.
向仅接受孕酮维持的去卵巢大鼠的每个子宫角注射12 - 5纳摩尔二丁酰环磷腺苷（dbcAMP），可增强[3H]亮氨酸掺入子宫腔蛋白的能力。注射dbcAMP后，每只大鼠[3H]亮氨酸掺入量为11 - 73×10³ 计数每分钟（c.p.m.），而注射生理盐水后为3 - 69×10³ c.p.m./大鼠。相比之下，注射1.0微克雌二醇后，每只大鼠为58.05×10³ c.p.m.。电泳分析表明，注射dbcAMP或雌二醇后发现的放射性标记蛋白在质量上与妊娠第5天（胚泡着床日）检测到的蛋白相似。因此，该核苷酸显然引发了与注射雌二醇后观察到的类似的子宫反应。
