通过逆转录聚合酶链反应（RT-PCR）快速检测热休克因子1（HSF1）和热休克因子2（HSF2）基因表达
Rapid assay of HSF1 and HSF2 gene expression by RT-PCR.
作者信息
Ding X Z, Smallridge R C, Galloway R J, Kiang J G
机构信息
Department of Clinical Physiology, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
出版信息
Mol Cell Biochem. 1996 May 24;158(2):189-92. doi: 10.1007/BF00225845.
Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.
按照本研究中所述的方案，设计了引物，以便能够快速且可靠地检测人表皮样A431细胞中的热休克转录因子1和2。使用这些引物，观察到热诱导后热休克转录因子1增加，而热休克转录因子2没有增加。这是第一份表明热休克增加HSF1的mRNA量而HSF2的mRNA量没有变化的报告。
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