Polla B S, Healy A M, Wojno W C, Krane S M
Am J Physiol. 1987 Jun;252(6 Pt 1):C640-9. doi: 10.1152/ajpcell.1987.252.6.C640.
Monocytes, which play a central role in inflammatory reactions, are exposed to elevated temperatures in febrile states or to potential cellular toxins (e.g., reactive oxygen species, immune mediators). We analyzed the heat shock (HS) response in human peripheral blood monocytes and the monocytic line U937 compared with that in dermal fibroblasts. After HS, U937 cells and fibroblasts synthesized mainly the 70- and 83-kDa HS proteins (HSPs), whereas the spectrum of HSPs synthesized by monocytes varied over the range 41-45 degrees C. Incubation with hydrogen peroxide induced synthesis of the 70-kDa HSP in monocytes only. The hormone 1 alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] that protects monocytes from thermal injury had similar effects on the HS response in U937 cells, increasing synthesis of HSPs and levels of HSP 70 mRNA and partially preventing the decrease in normal protein synthesis, whereas interferon-gamma had no effect. 1,25-(OH)2D3 did not modify the HS response in fibroblasts, although these cells contained cellular receptors for 1,25-(OH)2D3. 1,25-(OH)2D3 could play a specific role in modulating the HS response in monocytes and related cells.
在炎症反应中起核心作用的单核细胞，在发热状态下会暴露于升高的温度中，或接触潜在的细胞毒素（如活性氧、免疫介质）。我们分析了人类外周血单核细胞和单核细胞系U937中的热休克（HS）反应，并与真皮成纤维细胞中的反应进行了比较。热休克后，U937细胞和成纤维细胞主要合成70 kDa和83 kDa的热休克蛋白（HSP），而单核细胞合成的HSP谱在41 - 45摄氏度范围内有所不同。仅用过氧化氢孵育可诱导单核细胞中70 kDa HSP的合成。保护单核细胞免受热损伤的激素1α,25 - 二羟基维生素D3 [1,25-(OH)2D3] 对U937细胞中的HS反应有类似影响，增加HSP的合成以及HSP 70 mRNA的水平，并部分防止正常蛋白质合成的减少，而干扰素 - γ则无此作用。尽管成纤维细胞含有1,25-(OH)2D3的细胞受体，但1,25-(OH)2D3并未改变其HS反应。1,25-(OH)2D3可能在调节单核细胞及相关细胞的HS反应中发挥特定作用。
