Budker V, Zhang G, Knechtle S, Wolff J A
Department of Pediatrics, Waisman Center, University of Wisconsin-Madison 53705, USA.
Gene Ther. 1996 Jul;3(7):593-8.
Naked plasmid DNA in hypertonic solutions was injected intraportally in mice whose hepatic veins were transiently occluded. High levels of luciferase expression and beta-galactosidase expression in 1% of the hepatocytes throughout the entire liver were achieved using 100 micrograms of the respective plasmid vector. Two days after the intraportal injection of 100 micrograms of pCMVGH, the mean hGH serum concentration was 65 ng/ml +/- 26 (n = 7) which is approximately 50-fold above normal baseline levels. These unprecedented levels of foreign gene expression from naked plasmid DNA document the ability of parenchymal cells in vivo to take up naked DNA following intravascular delivery.
将处于高渗溶液中的裸质粒DNA经门静脉注射到肝静脉被短暂闭塞的小鼠体内。使用100微克相应的质粒载体，可使整个肝脏中1%的肝细胞实现高水平的荧光素酶表达和β-半乳糖苷酶表达。在经门静脉注射100微克pCMVGH两天后，血清中人促生长激素（hGH）的平均浓度为65纳克/毫升±26（n = 7），约为正常基线水平的50倍。裸质粒DNA所实现的这些前所未有的外源基因表达水平证明了实质细胞在血管内递送后在体内摄取裸DNA的能力。
