Perez S E, Adrio F, Rodriguez M A, Rodriguez-Moldes I, Anadon R
Department of Fundamental Biology, Faculty of Biology, University of Santiago de Compostela, Spain.
Neurosci Lett. 1996 Feb 23;205(2):83-6. doi: 10.1016/0304-3940(96)12379-x.
We studied the distribution of NADPH-diaphorase (NADPHd) in the brain of the rainbow trout. Not only neurons but also tanycytes and oligodendrocytes showed NADPHd positivity. Interestingly, staining of oligodendrocytes was delicate and revealed fine characteristics of these cells, comparable to those revealed by classical 'oligodendrocyte methods' in mammals (such as the rich branching of processes and their association with myelin sheaths). NADPHd histochemistry indicated that trout oligodendrocytes are heterogeneous as regards cell size, shape and number of processes, and that there is a positive correlation between the size of axons and that of the associated oligodendrocytes. NADPHd histochemistry thus appears to be a useful method for the study of oligodendrocyte populations in bony fishes. Furthermore, this is the first report of NADPHd activity in oligodendrocytes of any vertebrate.
我们研究了虹鳟鱼大脑中NADPH-黄递酶（NADPHd）的分布。不仅神经元，而且伸长细胞和少突胶质细胞都显示出NADPHd阳性。有趣的是，少突胶质细胞的染色很精细，揭示了这些细胞的精细特征，这与哺乳动物中经典的“少突胶质细胞方法”所揭示的特征相当（例如丰富的分支突起及其与髓鞘的关联）。NADPHd组织化学表明，鳟鱼少突胶质细胞在细胞大小、形状和突起数量方面存在异质性，并且轴突大小与相关少突胶质细胞大小之间存在正相关。因此，NADPHd组织化学似乎是研究硬骨鱼少突胶质细胞群体的一种有用方法。此外，这是关于任何脊椎动物少突胶质细胞中NADPHd活性的首次报道。
