Suyama Y, Kawamuro K, Kinoshita I, Yoshimura K, Tsumura Y, Takahara H
Bio-resources Technology Division, Forestry and Forest Products Research Institute, Ibaraki, Japan.
Genes Genet Syst. 1996 Jun;71(3):145-9. doi: 10.1266/ggs.71.145.
DNA was amplified from individual fossil pollen grains of Abies spp. (Pinaceae), which have been detected from Pleistocene peaty deposits (at least 150,000 years old). To identify the species of the fossil pollen by DNA analysis, the region indicating the species-specific sequence was searched among extant Abies species and the spacer region between rrn5 and trnR in chloroplast DNA was sequenced for four grains of the fossil pollen. Three pollen samples produced the same sequence as extant Abies species. The sequence for the remaining sample differed from that of extant Abies by one substitution. This study showed not only a successful DNA analysis from a single grain of fossil pollen but also a new method to identify the species of fossil pollen for the pollen analysis field.
从冷杉属（松科）的单个化石花粉粒中扩增出了DNA，这些花粉粒是从更新世泥炭沉积物（至少有15万年历史）中检测到的。为了通过DNA分析鉴定化石花粉的物种，在现存冷杉物种中搜索了指示物种特异性序列的区域，并对四颗化石花粉粒的叶绿体DNA中rrn5和trnR之间的间隔区进行了测序。三个花粉样本产生了与现存冷杉物种相同的序列。其余样本的序列与现存冷杉的序列有一个替换差异。这项研究不仅展示了从单个化石花粉粒成功进行DNA分析，还为花粉分析领域提供了一种鉴定化石花粉物种的新方法。
