Ferenczy A, Franco E, Arseneau J, Wright T C, Richart R M
Department of Pathology, McGill University, Montreal, Quebec, Canada.
Am J Obstet Gynecol. 1996 Sep;175(3 Pt 1):651-6. doi: 10.1053/ob.1996.v175.a73868.
Our purpose was to determine the diagnostic performance of human papillomavirus deoxyribonucleic acid testing when performed on liquid-based (Thin-prep) cytologic samples.
Thin-prep cytologic study, human papillomavirus deoxyribonucleic acid assay with probes for high oncogenic risk human papillomavirus types (16, 18, 31, 33, 35, 45, 51, 52, and 56), and cervical biopsies on women referred to colposcopy for an abnormal Papanicolaou smear were performed.
Of the 364 patients, 186 (51.6%) had a low-grade squamous intraepithelial lesion or worse by histologic diagnosis. Human papillomavirus deoxyribonucleic acid quantitation was correlated with a histologic diagnosis of squamous intraepithelial lesion (p < 0.0001) and the morphologic severity (p < 0.01). The combination of Thin-prep cytologic study and human papillomavirus deoxyribonucleic acid testing correctly identified 87.7% of histologic low-grade lesions and 95.1% of the women with high grade lesions and invasive cancer (p < 0.01).
A liquid-based cytologic system provides adequate material for concomitant human papillomavirus testing. Addition of human papillomavirus testing to Thin-prep cytologic study provides significant gains in diagnostic accuracy over either cytologic study or human papillomavirus deoxyribonucleic acid testing alone.
我们的目的是确定在液基（薄层液基）细胞学样本上进行人乳头瘤病毒脱氧核糖核酸检测的诊断性能。
对因巴氏涂片异常而转诊至阴道镜检查的女性进行薄层液基细胞学研究、使用针对高致癌风险人乳头瘤病毒类型（16、18、31、33、35、45、51、52和56）的探针进行人乳头瘤病毒脱氧核糖核酸检测以及宫颈活检。
在364例患者中，186例（51.6%）经组织学诊断为低度鳞状上皮内病变或更严重病变。人乳头瘤病毒脱氧核糖核酸定量与鳞状上皮内病变的组织学诊断（p < 0.0001）和形态学严重程度（p < 0.01）相关。薄层液基细胞学研究与人乳头瘤病毒脱氧核糖核酸检测相结合，正确识别出87.7%的组织学低度病变以及95.1%的高级别病变和浸润癌女性患者（p < 0.01）。
液基细胞学系统为同时进行人乳头瘤病毒检测提供了充足的样本。在薄层液基细胞学研究中加入人乳头瘤病毒检测，相比于单独进行细胞学研究或人乳头瘤病毒脱氧核糖核酸检测，在诊断准确性方面有显著提高。
