Bianchi Anne, Moret François, Desrues Jean-Marc, Champenois Thierry, Dervaux Yves, Desvouas Orlane, Oursin André, Quinzat Dominique, Dachez Roger, Bathelier Christian, Ronsin Christophe
Laboratoire Départemental de Seine-Saint-Denis, Conseil Général, Bondy, France.
J Clin Microbiol. 2002 May;40(5):1749-54. doi: 10.1128/JCM.40.5.1749-1754.2002.
The commercial COBAS Amplicor CT/NG test (Roche Diagnostic Systems, Meylan, France) is a sensitive and specific method for detection of Chlamydia trachomatis infections. This test currently consists of using a nucleic acid amplification method to detect C. trachomatis in first-void urine specimens and in endocervical swabs collected in 2-sucrose-phosphate (2SP) transport medium. We conducted a prospective study to determine whether the automated COBAS Amplicor CT/NG test can detect C. trachomatis in cervical specimens collected in PreservCyt transport medium (ThinPrep Pap Test; Cytyc Corporation, Boxborough, Mass.). PreservCyt medium is used to preserve cervical samples before the preparation of ThinPrep slides. We collected 1,000 cervical specimens from young women (age range, 15 to 25 years) during routine Pap smear tests. Only specimens with normal cytology and in which the gynecologist found no clinical evidence of urogenital infections were selected. The samples were stored in PreservCyt transport medium at 15 to 20 degrees C. C. trachomatis was detected in 22 of the 1,000 cervical specimens that had been stored in PreservCyt. To confirm the positive samples, the test was repeated on new endocervical swab specimens collected in 2SP transport medium. Only 9 of the 22 positive patients agreed to undergo this control, but all 9 retested positive. To evaluate the influence of storage conditions on the sensitivity of the C. trachomatis PCR test, all of the positive samples were stored at 15 to 20 degrees C in PreservCyt transport medium and were retested every 2 weeks for 6 weeks. C. trachomatis was successfully amplified from all 22 specimens for the whole 6-week period. The prevalence of C. trachomatis infection was 2.2% in our study population. These results demonstrate that PreservCyt transport medium is a suitable transport medium for detection of C. trachomatis by the COBAS Amplicor CT/NG test. The ThinPrep Pap Test may enable gynecologists to monitor for both cervical lesions and C. trachomatis infections with a single endocervical specimen.
商业化的COBAS Amplicor CT/NG检测（罗氏诊断系统公司，法国梅兰）是一种检测沙眼衣原体感染的灵敏且特异的方法。该检测目前采用核酸扩增法，用于检测首次晨尿标本以及采集于2-蔗糖-磷酸盐（2SP）转运培养基中的宫颈拭子中的沙眼衣原体。我们进行了一项前瞻性研究，以确定自动化的COBAS Amplicor CT/NG检测能否检测采集于PreservCyt转运培养基（ThinPrep巴氏试验；Cytyc公司，马萨诸塞州博克斯伯勒）中的宫颈标本中的沙眼衣原体。PreservCyt培养基用于在制备ThinPrep玻片之前保存宫颈样本。我们在常规巴氏涂片检查期间，从年轻女性（年龄范围15至25岁）中收集了1000份宫颈标本。仅选择了细胞学正常且妇科医生未发现泌尿生殖系统感染临床证据的标本。样本保存在PreservCyt转运培养基中，温度为15至20摄氏度。在保存在PreservCyt中的1000份宫颈标本中，有22份检测出沙眼衣原体。为了确认阳性样本，对采集于2SP转运培养基中的新的宫颈拭子标本重复进行检测。22名阳性患者中只有9名同意接受此项对照检测，但所有9人再次检测均为阳性。为了评估储存条件对沙眼衣原体PCR检测敏感性的影响，所有阳性样本均保存在PreservCyt转运培养基中，温度为15至20摄氏度，并在6周内每2周重新检测一次。在整个6周期间，所有22份标本均成功扩增出沙眼衣原体。在我们的研究人群中，沙眼衣原体感染率为2.2%。这些结果表明，PreservCyt转运培养基是通过COBAS Amplicor CT/NG检测检测沙眼衣原体的合适转运培养基。ThinPrep巴氏试验可能使妇科医生能够用单个宫颈标本同时监测宫颈病变和沙眼衣原体感染。
