Rainey P B, Bailey M J
Department of Plant Sciences, University of Oxford, UK.
Mol Microbiol. 1996 Feb;19(3):521-33. doi: 10.1046/j.1365-2958.1996.391926.x.
Pseudomonas fluorescens is a saprophytic bacterium commonly isolated from soil, water, and the surfaces and tissues of plants and animals. The species has important applications in biotechnology because it can enhance plant growth and protect crops against disease. A complete physical map of the 6.63 Mbp P. fluorescens SBW25 chromosome was constructed using data obtained from combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA with end labelling. In total, 139 restriction sites (15 PacI, 53 SpeI, 71 XbaI) were placed on the physical map and complete maps of the circular chromosome were obtained for both PacI and SpeI; only XbaI fragments linking SpeI fragments were positioned. The average resolution of restriction sites was 48 kbp. A genetic map was derived from the physical map by southern hybridization and 31 genes were positioned including oriC, rDNA operons (rnnA-E), recA, gacA, and pyvD.
荧光假单胞菌是一种腐生细菌，通常从土壤、水以及动植物的表面和组织中分离得到。该物种在生物技术领域具有重要应用，因为它可以促进植物生长并保护作物免受病害侵袭。利用完全或部分消化的染色体DNA经末端标记后的一维和二维电泳组合数据，构建了6630kbp荧光假单胞菌SBW25染色体的完整物理图谱。总共在物理图谱上定位了139个限制酶切位点（15个PacI、53个SpeI、71个XbaI），并获得了PacI和SpeI的环状染色体完整图谱；仅定位了连接SpeI片段的XbaI片段。限制酶切位点的平均分辨率为48kbp。通过Southern杂交从物理图谱推导出遗传图谱，并定位了31个基因，包括oriC、rDNA操纵子（rnnA - E）、recA、gacA和pyvD。
