Benfield D A, Nelson E, Collins J E, Harris L, Goyal S M, Robison D, Christianson W T, Morrison R B, Gorcyca D, Chladek D
Department of Veterinary Science, South Dakota State University, Brookings 57007.
J Vet Diagn Invest. 1992 Apr;4(2):127-33. doi: 10.1177/104063879200400202.
The characterization of an isolate of swine infertility and respiratory syndrome (SIRS) virus (ATCC VR-2332) is reported. A commercial cell line (CL2621) was used for the propagation of the virus for all assays. Laboratory studies indicate that this isolate is a fastidious, nonhemagglutinating, enveloped RNA virus. Cesium chloride-purified virions visualized by electron microscopy were spherical particles with an average diameter of 62 nm (range: 48-83 nm) and a 25-30 nm core surrounded by an envelope. Virus replication was restricted to the cytoplasm, as demonstrated by immunofluorescence. The virus did not react serologically with antisera to several common porcine viruses or with antisera to known viruses in the alphavirus, rubivirus, pestivirus, and ungrouped lactic dehydrogenase virus genera of the Togaviridae. However, convalescent sow sera and rabbit hyperimmune sera neutralized the SIRS virus at titers of 1:256 and 1:512, respectively. The virus was stable at 4 and -70 C, but was labile at 37 and 56 C. The properties of this isolate of SIRS virus resemble those of the family Togaviridae but do not match the described genera.
本文报道了猪不育与呼吸综合征（SIRS）病毒（ATCC VR - 2332）分离株的特性。所有试验均使用一种商业细胞系（CL2621）来繁殖该病毒。实验室研究表明，该分离株是一种苛求、无血凝性、有包膜的RNA病毒。通过电子显微镜观察，经氯化铯纯化的病毒粒子为球形颗粒，平均直径62nm（范围：48 - 83nm），有一个25 - 30nm的核心，被包膜包围。免疫荧光显示病毒复制局限于细胞质。该病毒与针对几种常见猪病毒的抗血清以及针对披膜病毒科中甲病毒、风疹病毒、瘟病毒和未分类的乳酸脱氢酶病毒属已知病毒的抗血清均无血清学反应。然而，康复母猪血清和兔超免疫血清分别以1:256和1:512的效价中和了SIRS病毒。该病毒在4℃和 - 70℃稳定，但在37℃和56℃不稳定。这种SIRS病毒分离株的特性类似于披膜病毒科，但与已描述的属不相符。
