Moriyama R, Hattori A, Miyata S, Kudoh S, Makino S
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Aichi, Japan.
J Bacteriol. 1996 Oct;178(20):6059-63. doi: 10.1128/jb.178.20.6059-6063.1996.
The Bacillus subtilis sleB gene, which codes for the enzyme homologous to the germination-specific amidase from Bacillus cereus, was cloned and its nucleotide sequence was determined. Sequence analysis showed that it had an open reading frame of 918 bp, coding for a polypeptide of 305 amino acids with a putative signal sequence of 29 residues. Enzyme activity was not found in germination exudate of B. subtilis spores, which differs from the case of B. cereus enzyme. A B. subtilis mutant with an insertionally inactivated sleB gene revealed normal behavior in growth and sporulation. However, the sleB mutant was unable to complete germination mediated by L-alanine.
枯草芽孢杆菌的sleB基因编码一种与蜡样芽孢杆菌的萌发特异性酰胺酶同源的酶，该基因已被克隆并测定了其核苷酸序列。序列分析表明，它有一个918 bp的开放阅读框，编码一个由305个氨基酸组成的多肽，带有一个29个残基的推定信号序列。在枯草芽孢杆菌孢子的萌发渗出物中未发现酶活性，这与蜡样芽孢杆菌酶的情况不同。一个sleB基因插入失活的枯草芽孢杆菌突变体在生长和孢子形成方面表现出正常行为。然而，sleB突变体无法完成由L-丙氨酸介导的萌发。
