Sönnichsen B, Watson R, Clausen H, Misteli T, Warren G
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
J Cell Biol. 1996 Sep;134(6):1411-25. doi: 10.1083/jcb.134.6.1411.
COP I-coated vesicles were analyzed for their content of resident Golgi enzymes (N-acetylgalactosaminyltransferase; N-acetylglucosaminyltransferase I; mannosidase II; galactosyltransferase), cargo (rat serum albumin; polyimmunoglobulin receptor), and recycling proteins (-KDEL receptor; ERGIC-53/p58) using biochemical and morphological techniques. The levels of these proteins were similar when the vesicles were prepared under interphase or mitotic conditions showing that sorting was unaffected. The average density relative to starting membranes for resident enzymes (14-30%), cargo (16-23%), and recycling proteins (81-125%) provides clues to the function of COP I vesicles in transport through the Golgi apparatus.
利用生化和形态学技术分析了被COP I包被的囊泡，以检测其所含的高尔基体驻留酶（N-乙酰半乳糖胺基转移酶；N-乙酰葡糖胺基转移酶I；甘露糖苷酶II；半乳糖基转移酶）、货物（大鼠血清白蛋白；多聚免疫球蛋白受体）和循环蛋白（-KDEL受体；ERGIC-53/p58）。当在间期或有丝分裂条件下制备囊泡时，这些蛋白质的水平相似，表明分选未受影响。相对于起始膜，驻留酶（14 - 30%）、货物（16 - 23%）和循环蛋白（81 - 125%）的平均密度为COP I囊泡在通过高尔基体的运输中的功能提供了线索。
