Guisantes J A, Eraso E, Felleisen R, Martinez J, Gottstein B
Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, Vitoria, Spain.
J Investig Allergol Clin Immunol. 1996 Jan-Feb;6(1):65-8.
In this study, the conditions for the successful application of the random amplified polymorphic DNA (RAPD) assay to differentiate mite populations based on genetic variation were defined. Five species of mites related to allergic diseases were studied: Dermatophagoides pteronyssinus, D. farinae (2 strains), Blomia tropical is, Glycyphagus domesticus and Tyrophagus putrescentiae. The mites were isolated from pure cultures and processed according to the method described in this paper. The banding patterns obtained were different for all the species studied. When the DNA from two different strains of D. farinae were studied, the "fingerprint" banding patterns obtained showed differences between them. The random amplified polymorphic DNA assay may be a useful tool to aid the taxonomic study of mite populations.
在本研究中，确定了基于遗传变异成功应用随机扩增多态性DNA（RAPD）分析来区分螨种群的条件。研究了与过敏性疾病相关的五种螨：粉尘螨、屋尘螨（2个菌株）、热带无爪螨、家甜食螨和腐食酪螨。螨从纯培养物中分离出来，并按照本文所述方法进行处理。所研究的所有物种获得的条带模式均不同。当研究来自两种不同屋尘螨菌株的DNA时，获得的“指纹”条带模式显示出它们之间的差异。随机扩增多态性DNA分析可能是有助于螨种群分类学研究的有用工具。
