Bjartell A, Malm J, Moller C, Gunnarsson M, Lundwall A
Department of Urology, University Hospital, Malmo, Sweden.
J Androl. 1996 Jan-Feb;17(1):17-26.
Semenogelin I and II (Sgl, Sgll) are two separate gene products of chromosome 20 with extensive (80%) identity in primary structure. They are mainly responsible for immediate gel formation of freshly ejaculated semen. Degradation of Sgl and Sgll is due to the proteolytic action of prostate-specific antigen (PSA); it results within 5-15 minutes in liquefaction of semen and release of progressively motile spermatozoa. By means of cDNA cloning and Northern blots, Sgl and Sgll transcripts have previously been shown to be abundant in human seminal vesicles, but Sgll alone is suggested to be expressed at low levels in the epididymis. To characterize the expression and tissue distribution of Sgl and Sgll in greater detail, we produced monoclonal immunoglobulin Gs (lgGs for immunocytochemistry (lCC) and specific [35S]-, digoxigenin-, or alkaline phosphatase-labeled 30-mer antisense probes to Sgl and Sgll for in situ hybridization (lSH). Immunocytochemical staining for both Sgl and Sgll, and lSH detection of both Sgl and Sgll transcripts, were demonstrated in the cytoplasm of seminal vesicle epithelium. lSH showed Sgll alone to be expressed in the epithelium of the epididymal cauda. Neither lCC nor lSH yielded any evidence of Sgl or Sgll expression in caput or corpus epithelium or in any stromal cells of the epididymis. Consistent with our previous findings using polyclonal lgG, monoclonal anti-Sgll Sgll lgGs identified epitopes on the posterior head, midpiece, and tail of ejaculated spermatozoa. Spermatozoa in the epididymal cauda were also immunoreactive, but those in the caput or corpus region of the epididymis as well as those in the testis were negative. As shown by lCC, neither Sgl nor Sgll were expressed in the testis, the prostate, the female genital tract, or other normal human tissue specimens. Although the significance of Sg attachment to epididymal and ejaculated spermatozoa remains to be established, monoclonal anti-Sg lgG might prove useful in establishing the origin of seminal vesicle tissue components in prostate core biopsies or other biopsy specimens.
精液凝固蛋白I和II（Sgl、Sgll）是20号染色体上两个独立的基因产物，其一级结构具有广泛的（80%）同源性。它们主要负责刚射出精液的快速凝胶形成。Sgl和Sgll的降解是由于前列腺特异性抗原（PSA）的蛋白水解作用；这会在5 - 15分钟内导致精液液化并释放出具有渐进运动能力的精子。通过cDNA克隆和Northern印迹分析，先前已表明Sgl和Sgll转录本在人精囊中大量存在，但仅Sgll被认为在附睾中低水平表达。为了更详细地描述Sgl和Sgll的表达及组织分布，我们制备了单克隆免疫球蛋白G（用于免疫细胞化学（lCC）的lgG）以及针对Sgl和Sgll的特异性[35S] -、地高辛或碱性磷酸酶标记的30聚体反义探针用于原位杂交（lSH）。在精囊上皮细胞的细胞质中证实了对Sgl和Sgll两者的免疫细胞化学染色以及对Sgl和Sgll转录本两者的lSH检测。lSH显示仅Sgll在附睾尾部上皮中表达。lCC和lSH均未提供Sgl或Sgll在附睾头或体部上皮或附睾任何基质细胞中表达的证据。与我们先前使用多克隆lgG的发现一致，单克隆抗Sgll Sgll lgG识别射出精子的后头部、中段和尾部的表位。附睾尾部的精子也具有免疫反应性，但附睾头或体部区域以及睾丸中的精子呈阴性。如lCC所示，Sgl和Sgll在睾丸、前列腺、女性生殖道或其他正常人体组织标本中均未表达。尽管Sg附着于附睾和射出精子的意义仍有待确定，但单克隆抗Sg lgG可能在确定前列腺核心活检或其他活检标本中精囊组织成分的来源方面有用。
