Clark D A, Delcros J G, Craft I
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
J Assist Reprod Genet. 1996 Jul;13(6):464-7. doi: 10.1007/BF02066525.
Our purpose was to test the prediction that spermine levels in IVF culture supernatants measure by rapid ELISA assay correlate with subsequent success or failure to establish a pregnancy.
Forty-nine patients undergoing gonadotropin-stimulated ovulation after LHRH analogue treatment in the current study succeeded and 57 failed. With the exception of the first 4 hr of culture, where polyamine levels were slightly (but not significantly) higher in supernatants associated with subsequent success, higher levels of polyamines were predictive of failure.
The result in this study differs from previous data obtained with women ovulated using clomiphene where low spermine levels (assayed in an immunosuppression assay in vitro) correlated with failure to establish pregnancy, and detectable levels correlated with success. Supplementation of IVF culture medium with spermine and/or spermidine appears unlikely to improve IVF success rates, where the success rate is already very good, and may possibly do harm. The possibility that different methods of ovulation affect subsequent polyamine production in vitro by fertilized oocytes merits further study.
我们的目的是检验一项预测，即通过快速酶联免疫吸附测定法检测的体外受精培养液上清液中的精胺水平与随后妊娠的成功或失败相关。
在本研究中，49例接受促性腺激素释放激素类似物治疗后进行促性腺激素刺激排卵的患者成功妊娠，57例失败。除培养的前4小时外，与随后成功相关的上清液中的多胺水平略高（但不显著），较高水平的多胺预示着失败。
本研究结果与先前使用克罗米芬促排卵的女性的数据不同，在先前研究中，低精胺水平（在体外免疫抑制测定中检测）与妊娠失败相关，可检测水平与成功相关。在体外受精成功率已经很高的情况下，向体外受精培养液中添加精胺和/或亚精胺似乎不太可能提高成功率，甚至可能有害。不同的排卵方法是否会影响受精卵在体外随后的多胺产生，这一可能性值得进一步研究。
