Sato K, Akiba Y, Kimura S, Horiguchi M
Department of Animal Science, Tohoku University, Sendaishi, Japan.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1995 Nov;112(3):315-9. doi: 10.1016/0742-8413(95)02026-8.
To identify the species differences in lipoprotein hydrolysis, plasma lipoproteins and lipoprotein lipase (LPLase) prepared from both rats and chicks were incubated in vitro in the presence of Triton WR-1339 at concentrations up to 500 micrograms/ml. Rate of lipoprotein hydrolysis by LPLase declined gradually with an increase of Triton concentration irrespective of differences in sources in the lipoprotein and LPLase. At 250 micrograms/ml Triton, the lipoprotein hydrolysis was inhibited by 90% in rats but was only inhibited by < 20% in chicks. Lipoprotein hydrolysis inhibited by Triton was partly recovered by an increase of LPLase concentration, and extents of the recovery were prominent either when rat lipoprotein was provided in place of chick lipoprotein and when rat LPLase rather than chick LPLase was used as the enzyme source for each lipoprotein. These results suggest species differences between chicks and rats with regard to the affinity of Triton to LPLase and lipoprotein and/or the chemical characteristics of both LPLase and lipoprotein.
为了确定脂蛋白水解的种属差异，分别从大鼠和雏鸡制备的血浆脂蛋白和脂蛋白脂肪酶（LPLase）在含有浓度高达500微克/毫升的 Triton WR - 1339的体外环境中孵育。无论脂蛋白和LPLase来源的差异如何，LPLase引起的脂蛋白水解速率都随着Triton浓度的增加而逐渐下降。在250微克/毫升的Triton浓度下，大鼠的脂蛋白水解被抑制了90%，而雏鸡的脂蛋白水解仅被抑制了不到20%。Triton抑制的脂蛋白水解可通过增加LPLase浓度而部分恢复，当用大鼠脂蛋白替代雏鸡脂蛋白以及当使用大鼠LPLase而非雏鸡LPLase作为每种脂蛋白的酶源时，恢复程度都很显著。这些结果表明，在Triton对LPLase和脂蛋白的亲和力以及LPLase和脂蛋白的化学特性方面，雏鸡和大鼠之间存在种属差异。
