Huang M E, Manus V, Chuat J C, Galibert F
UPR41-CNRS Recombinaisons Génétiques, Faculté de Médecine, Rennes, France.
Yeast. 1996 Jul;12(9):869-75. doi: 10.1002/(SICI)1097-0061(199607)12:9%3C869::AID-YEA964%3E3.0.CO;2-1.
We have sequenced a 61.989 bp stretch located between genes RAD7 and FIP1 of Saccharomyces cerevisiae chromosome X. This stretch contains 36 open reading frames (ORFs) of at least 100 codons. Fourteen of these correspond to sequences previously published as HIT1, CDC8, YAP17, CBF1, NAT1, RPA12, CCT5, TOR1, RFC2, PEM2, CDC11, MIR1, STE18 and GRR1. The proteins deduced from four ORFs (YJR059w, YJR065c, YJR075w, YJR078w) have significant similarity to proteins of known function from yeast or other organisms, including S. cerevisiae serine/threonine-specific protein kinase. Schizosaccharomyces pombe Act2 protein, S. cerevisiae mannosyltransferase OCH1 protein and mouse indoleamine 2,3-dioxygenase, respectively. Four of the remaining 18 ORFs have similarity to proteins with unknown function, six are weakly similar to other known sequences, while another eight exhibit no similarity to any known sequence. In addition, three tRNA genes have been recognized. Three genes clustered within 22 kb (YJR059w, YJR061w and TOR1) have counterparts arranged within 15 kb on the left arm of chromosome XI.
我们对酿酒酵母X染色体上位于RAD7基因和FIP1基因之间的一段61989 bp的序列进行了测序。这段序列包含36个至少有100个密码子的开放阅读框（ORF）。其中14个与先前发表的HIT1、CDC8、YAP17、CBF1、NAT1、RPA12、CCT5、TOR1、RFC2、PEM2、CDC11、MIR1、STE18和GRR1序列相对应。从4个ORF（YJR059w、YJR065c、YJR075w、YJR078w）推导的蛋白质与酵母或其他生物体中已知功能的蛋白质具有显著相似性，包括酿酒酵母丝氨酸/苏氨酸特异性蛋白激酶、粟酒裂殖酵母Act2蛋白、酿酒酵母甘露糖基转移酶OCH1蛋白和小鼠吲哚胺2,3-双加氧酶。其余18个ORF中的4个与功能未知的蛋白质相似，6个与其他已知序列有弱相似性，而另外8个与任何已知序列均无相似性。此外，还识别出3个tRNA基因。在22 kb范围内成簇的3个基因（YJR（）59w、YJR061w和TOR1）在XI号染色体左臂上15 kb范围内有对应物。
