Ironside J, Faed M J
Hum Genet. 1979 Apr 17;48(1):39-43. doi: 10.1007/BF00273272.
Mitotic preparations from 30 subfertile males and meiotic preparations from 3 normal and 2 subfertile males were examined by means of the Ag-I technique of Bloom and Goodpasture (1976) to reveal nucleolus organiser regions (NORs). In the mitotic preparations, each subject was found to have a characteristic number of Ag-positive NORs per cell, within a range of 6--10. Analysis of satellite associations showed that the mean number of satellite associations per cell was related to the modal number of Ag-positive NORs for each subject. In the meiotic preparations, silver deposition was observed throughout meiotic prophase, but disappeared totally during diakinesis and metaphase II. It was seen again in early spermatids, and disappeared again as nuclear elongation took place. This pattern was observed in both normal and subfertile subjects, and may provide indirect evidence for the activation of rRNA genes during spermatogenesis.
采用布鲁姆和古德帕斯特（1976年）的银染技术对30名不育男性的有丝分裂标本以及3名正常男性和2名不育男性的减数分裂标本进行检查，以显示核仁组织区（NORs）。在有丝分裂标本中，发现每个受试者每个细胞的银染阳性NORs数量具有特征性，范围在6 - 10个之间。对随体联合的分析表明，每个细胞的随体联合平均数与每个受试者银染阳性NORs的众数相关。在减数分裂标本中，整个减数分裂前期都观察到银沉积，但在终变期和减数第二次分裂中期完全消失。在早期精子细胞中又再次出现，并随着细胞核伸长再次消失。在正常和不育受试者中均观察到这种模式，这可能为精子发生过程中rRNA基因的激活提供间接证据。
