Leygue E, Snell L, Hiller T, Dotzlaw H, Hole K, Murphy L C, Watson P H
Department of Biochemistry and Molecular Biology, University of Manitoba, Faculty of Medicine, Winnipeg, Canada.
Cancer Res. 1996 Oct 15;56(20):4606-9.
The mRNA encoding the calcium-binding protein psoriasin was identified as being more highly expressed in the in situ versus the invasive component of the same breast tumor. Reverse transcription-PCR analysis of total RNA extracted from three independent cases of ductal carcinoma in situ of the comedo type and three cases of high-grade invasive ductal carcinoma revealed a detectable level of psoriasin mRNA expression in the in situ lesions only. Similar analysis performed on total RNA extracted from frozen sections of 32 independent breast samples, representing a continuum from normal to invasive tumor, confirmed high psoriasin expression in ductal carcinoma in situ of the comedo type only. The possible functional role of the psoriasin protein in breast tumor cells remains to be determined.
编码钙结合蛋白银屑素的信使核糖核酸（mRNA）被确定在同一乳腺肿瘤的原位成分中比浸润成分表达更高。对从3例独立的粉刺型原位导管癌和3例高级别浸润性导管癌中提取的总RNA进行逆转录聚合酶链反应（RT-PCR）分析，结果显示仅在原位病变中可检测到银屑素mRNA表达水平。对从32个独立乳腺样本的冰冻切片中提取的总RNA进行类似分析，这些样本代表了从正常到浸润性肿瘤的连续过程，结果证实仅在粉刺型原位导管癌中有高银屑素表达。银屑素蛋白在乳腺肿瘤细胞中的可能功能作用仍有待确定。
