Ochsner M
Ciba-Geigy Ltd., Physics Department, Basle, Switzerland.
Experientia. 1996 Sep 15;52(9):856-64. doi: 10.1007/BF01938870.
The dose-dependent effect of CGP 45715A on the LTD4-induced Ca2+ response of glomerular mesangial cells has been studied. Our results demonstrate that the LTD4-dependent increase in the cytosolic Ca2+ concentration primarily involves an InsP3-mediated release of Ca2+ from intracellular storage sites and to a minor extent an enhanced influx of Ca2+ through receptor-operated Ca2+ channels located in the plasma membrane. The action of CGP 45715A on the Ca2+ response is an inhibitory one and is convincingly explained by a displacement of LTD4 from its receptor site(s). The contractile effect of LTD4 on pulmonary smooth muscle is proposed to be mainly caused by a receptor-mediated hydrolysis of phosphatidylinositol-4,5-bisphosphate.
已对CGP 45715A对白三烯D4（LTD4）诱导的肾小球系膜细胞Ca2+反应的剂量依赖性效应进行了研究。我们的结果表明，LTD4依赖性细胞溶质Ca2+浓度增加主要涉及肌醇三磷酸（InsP3）介导的细胞内储存部位Ca2+释放，在较小程度上还涉及通过位于质膜上的受体操纵性Ca2+通道增强的Ca2+内流。CGP 45715A对Ca2+反应的作用是抑制性的，并且可以通过LTD4从其受体位点的置换得到令人信服的解释。LTD4对肺平滑肌的收缩作用被认为主要是由受体介导的磷脂酰肌醇-4,5-二磷酸水解引起的。
