Miyake H, Yabu H, Satoh T, Yamamoto I
Department of Biological Sciences, Faculty of Science, Hiroshima University, Japan.
Nucleic Acids Symp Ser. 1995(34):91-2.
The mod genes coding for molybdenum transport system were isolated from Escherichia coli by means of complementation of the tor mutation that causes no synthesis of some molybdenum-containing enzymes. The nucleotide sequence of 3048-base pair showed four open reading frames including the previously reported modC gene. The genes were named modA, modB, modC, and modD. Promoter analysis by lacZ assay and primer extension showed the genes modA and modB individually have their own promoters. No transcriptional regulation of the two genes were observed in the presence of molybdate, nitrate, and oxygen in E. coli strain DH5 alpha.
通过对导致某些含钼酶无法合成的tor突变进行互补，从大肠杆菌中分离出编码钼转运系统的mod基因。3048个碱基对的核苷酸序列显示有四个开放阅读框，包括先前报道的modC基因。这些基因被命名为modA、modB、modC和modD。通过lacZ检测和引物延伸进行的启动子分析表明，基因modA和modB分别有各自的启动子。在大肠杆菌DH5α菌株中，在钼酸盐、硝酸盐和氧气存在的情况下，未观察到这两个基因的转录调控。
