日本嗜盐菌TR-1株细胞表面糖蛋白编码基因的克隆与测序。

Cloning and sequencing of the gene encoding the cell surface glycoprotein of Haloarcula japonica strain TR-1.

作者信息

Wakai H, Nakamura S, Kawasaki H, Takada K, Mizutani S, Aono R, Horikoshi K

机构信息

Department of Bioengineering, Tokyo Institute of Technology, Kanagawa, Japan.

出版信息

Extremophiles. 1997 Feb;1(1):29-35. doi: 10.1007/s007920050012.

DOI:10.1007/s007920050012

PMID:9680334

Abstract

The triangular disk-shaped halophilic archaeon Haloarcula japonica strain TR-1 has a glycoprotein on its cell surface. The complete gene encoding the cell surface glycoprotein (CSG) was cloned and sequenced. The gene has an open reading frame of 2586 bp, and a potential archaeal promoter sequence approximately 150 bp upstream of the ATG initiation codon. The mature CSG is composed of 828 amino acids and is preceded by a signal sequence of 34 amino acid residues. A hydropathy analysis showed a hydrophobic stretch at the C-terminus, that probably serves as a transmembrane domain. The amino acid sequence of the Ha. japonica CSG showed 52.1% and 43.2% identities to those from the Halobacterium halobium and Haloferax volcanii CSGs, respectively. Five potential N-glycosylation sites were found in the mature Ha. japonica CSG, sites that were distinctly different from those in Hb. halobium and Hf. volcanii. The Ha. japonica CSG gene was expressed in Escherichia coli.

摘要

三角形盘状嗜盐古菌日本嗜盐碱杆菌菌株TR-1在其细胞表面有一个糖蛋白。编码细胞表面糖蛋白（CSG）的完整基因被克隆并测序。该基因有一个2586 bp的开放阅读框，在ATG起始密码子上游约150 bp处有一个潜在的古菌启动子序列。成熟的CSG由828个氨基酸组成，前面有一个34个氨基酸残基的信号序列。亲水性分析显示在C端有一个疏水区域，可能作为跨膜结构域。日本嗜盐碱杆菌CSG的氨基酸序列与盐生盐杆菌和沃氏嗜盐碱杆菌CSG的氨基酸序列分别有52.1%和43.2%的同源性。在成熟的日本嗜盐碱杆菌CSG中发现了五个潜在的N-糖基化位点，这些位点与盐生盐杆菌和沃氏嗜盐碱杆菌中的位点明显不同。日本嗜盐碱杆菌CSG基因在大肠杆菌中表达。