Sakata Y, Fujimoto K, Ogata S, Koyama T, Fukagawa K, Sakai T, Tso P
Department of Internal Medicine, Saga Medical School, Japan.
Am J Physiol. 1996 Sep;271(3 Pt 1):G438-42. doi: 10.1152/ajpgi.1996.271.3.G438.
We examined satiation after intraduodenal infusion of triglyceride with or without Pluronic L-81 (L-81) in rats. L-81 suppresses lipid transport into mesenteric lymph by preventing the formation of chylomicrons. Under unrestrained conditions, a lipid emulsion was infused into 24-h fasted rats intraduodenally for 8 h. After the infusion, food intake during the first 30 min and blood samples were measured. Food intake after the 8-h lipid infusion decreased significantly (-75%) compared with that of rats infused with vehicle only, and the effect of the lipid meal was time dependent. The satiation effect of the lipid was lost as a result of L-81 treatment, but food intake was still significantly decreased (-22%). The loss of inhibition of food intake by the feeding of lipid plus L-81 was totally reversed by the cessation of L-81 infusion. In rats infused with the lipid emulsion, plasma levels of triglyceride and apolipoprotein A-IV increased significantly. This increase was abolished by the presence of L-81 in the lipid infusate. The present study indicates that postabsorptive factors are important for anorexia observed after a lipid meal in addition to preabsorptive factors. This study further supports our previous conclusion that apolipoprotein A-IV is an important postabsorptive factor for satiation after a lipid meal.
我们研究了在大鼠十二指肠内输注甘油三酯（无论有无普流罗尼克L-81（L-81））后的饱腹感情况。L-81通过阻止乳糜微粒的形成来抑制脂质转运至肠系膜淋巴。在无限制条件下，将脂质乳剂十二指肠内输注给禁食24小时的大鼠，持续8小时。输注后，测量最初30分钟内的食物摄入量并采集血样。与仅输注赋形剂的大鼠相比，8小时脂质输注后的食物摄入量显著降低（-75%），且脂质餐的作用具有时间依赖性。L-81处理导致脂质的饱腹感效应消失，但食物摄入量仍显著降低（-22%）。停止输注L-81后，脂质加L-81喂养对食物摄入抑制作用的丧失完全逆转。在输注脂质乳剂的大鼠中，血浆甘油三酯和载脂蛋白A-IV水平显著升高。脂质输注液中存在L-81可消除这种升高。本研究表明，除了吸收前因素外，吸收后因素对于脂质餐后出现的厌食也很重要。本研究进一步支持了我们之前的结论，即载脂蛋白A-IV是脂质餐后饱腹感的重要吸收后因素。
