Rogina B, Helfand S L
Department of Biostructure and Function, School of Dental Medicine, University of Connecticut Health Center, Farmington 06030, USA.
Genetics. 1996 Aug;143(4):1643-51. doi: 10.1093/genetics/143.4.1643.
The examination of beta-galactosidase (beta-gal) expression in the third segment of the antenna of the 2216 enhancer trap line in Drosophila melanogaster reveals two distinct spatial and temporal regulatory patterns of expression during adult life. Type 1 expression is characterized by a decline in the level of beta-gal expression with increasing age. Starting from a maximal level of expression at the time of adult emergence, there is a decrease in the number of cells that express beta-gal so that by 40-50 days of adult life few cells express beta-gal. Varying the ambient temperature and using hyperactivity mutants (Hyperkinetic, Shaker) demonstrates that the rate of this decline is independent of temperature and metabolic rate. Type II expression is distinctly different in spatial distribution and temporal regulation from the first pattern. Type II expression is restricted in the antenna to a small (< 20-30) set of cells whose level of expression changes in a periodic manner with time. The regulation of this periodicity appears to be linked to ambient temperature.
对黑腹果蝇2216增强子捕获系触角第三节中β-半乳糖苷酶（β-gal）表达的检测揭示了成虫期两种不同的时空表达调控模式。1型表达的特征是随着年龄增长β-gal表达水平下降。从成虫羽化时的最大表达水平开始，表达β-gal的细胞数量减少，以至于到成虫40 - 50天时，很少有细胞表达β-gal。改变环境温度并使用多动突变体（多动、颤抖）表明，这种下降速率与温度和代谢率无关。2型表达在空间分布和时间调控上与第一种模式明显不同。2型表达在触角中局限于一小群（<20 - 30个）细胞，其表达水平随时间呈周期性变化。这种周期性的调控似乎与环境温度有关。
