Andrews J, Smith M, Merakovsky J, Coulson M, Hannan F, Kelly L E
Department of Genetics, University of Melbourne, Victoria, Australia.
Genetics. 1996 Aug;143(4):1699-711. doi: 10.1093/genetics/143.4.1699.
The stoned gene of Drosophila melanogaster is required for normal neuronal function in both adult and larva. We have identified DNA sequences that lie within a genetic region that is known to include the stoned gene and that also reveal restriction site variations in two stoned lethal mutants. This genomic region contains a single transcription unit coding for an approximately 8.4-kb transcript. The transcript is preferentially expressed in the head of adult flies. The isolation and sequencing of cDNA and genomic clones reveals that stoned appears to encode a dicistronic mRNA, although the possible existence of other forms of mRNA cannot be excluded. Antibody cross-reactivity shows that two proteins are translated from the stoned locus in vivo. Both open reading frames (ORFs) encode novel proteins. The protein encoded by the first ORF contains four tandemly repeated motifs, and one domain of the protein encoded by the second ORF shows similarity to a family of proteins (AP50s) associated with clathrin assembly protein complexes.
黑腹果蝇的stoned基因在成虫和幼虫的正常神经元功能中都是必需的。我们已经鉴定出位于一个已知包含stoned基因的遗传区域内的DNA序列，这些序列还揭示了两个stoned致死突变体中的限制性位点变异。这个基因组区域包含一个单一的转录单元，编码一个约8.4kb的转录本。该转录本在成年果蝇的头部优先表达。cDNA和基因组克隆的分离与测序表明，stoned似乎编码一种双顺反子mRNA，尽管不能排除其他形式mRNA的存在。抗体交叉反应表明，体内有两种蛋白质从stoned基因座翻译而来。两个开放阅读框（ORF）都编码新的蛋白质。第一个ORF编码的蛋白质包含四个串联重复基序，第二个ORF编码的蛋白质的一个结构域与一组与网格蛋白组装蛋白复合物相关的蛋白质（AP50s）相似。
